Abl (H396P) active 10ug

Code: 14-750 D2-231

Application

Research Sub CategoryCardiovascular DiseaseMetabolic Disorders

Research CategoryMetabolism

Biochem/physiol Actions

Target Sub-F...


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Your Price
€486.20 EACH
Discontinued
€598.03 inc. VAT

Application

Research Sub CategoryCardiovascular DiseaseMetabolic Disorders

Research CategoryMetabolism

Biochem/physiol Actions

Target Sub-Family: TK

Protein Target: Abl

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Abl tyrosine kinase inhibitor STI571 (Gleevec™) is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse, due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the H396P mutation, which confers resistance to drug binding due to steric interference. (Corbin AS. et al, Blood, (2002); 101:4611-4614 and La Rosee P. et al, Cancer Research; 62:7149-7153).

Product Source: N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the H396P mutation. Expressed by baculovirus in Sf21 insect cells.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250µg size as catalog # 14-750M - Call for pricing and availability.

Physical form

50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.

Ni2+/NTA-agarose

Quality

Routinely evaluated by phosphorylation of Abltide.

Storage and Stability

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

biological sourcehuman
manufacturer/tradenameUpstate®
mol wtMw 121 kDa
Quality Level300, 100
recombinantexpressed in baculovirus infected Sf21 cells
technique(s)activity assay: suitable (kinase)
UniProt accession no.P00519, U07563
This product has met the following criteria to qualify for the following awards:



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